Not really I mean Zalcitabine the very toxic aids drug is basicly converted in T cells to ddCTP which blocks HIV reverse transcription by terminating the reverse transcribing HIV DNA.
https://en.m.wikipedia.org/wiki/Zalcitabine That's when the active ddCTP is only really found in one cell type. It's not something you want floating round your body.
If you go to buy some ddNTPs and look at the safety sheet you'll mostly see not tested or not known or some variation under most headings. I'm lead to believe it's pretty toxic. As a student they let me handle some fairly toxic stuff but they never let us touch that.
Be that as it may the dNTP-3'-OPO
3 would have other uses. You can basicly forget any chance of getting hold of highly patented reversible chain terminating dNTPs for diybio use. It's hard enough to get ordinary dNTPs. Diy bio is building a culture of self reliance and ultimately this is going to mean doing some chemistry for our selves. Well a few of us who feel brave will and the community as a whole will benefit.
Which brings us back to putting phosphate (PO
4) groups onto the 3' groups of dNTPs. The reagents used in 5' phosphorylation in the phosphoramidite method are Complex organic molecules that seem hard to acquire to me and probably very hard to synthesise. But the other issue is in the phosphoramidite method the DNA has protecting groups. I am concerned the standard reagents might interfere with the structure of unprotected nucleotides. Tbh I don't understand their chemistry enough to know if they would.
I'd like to know if they could be used but on balance I'm looking at processes which only involves access to well known simple compounds.