Sorry for the delay in my response.
If I understand correctly the units of Vmax in the PLoS Comp Bio reference is given in micromoles/min/kg body weight.I didn't pay attention to this information before,which is why the kcat that I calculated was too low.
I believe,in vitro Vmax has been converted to in vivo Vmax.(ref:New technologies for toxicity testing / edited by Michael Balls, Robert D. Combes, Nirmala Bhogal.)
Vmax (in vivo) = Vmax (in vitro) x Cprot x Ftiss
Vmax (in vivo) is maximal velocity of metabolism in vivo (mg/min per kg body weight),
Vmax (in vitro) is maximal velocity of metabolism in vitro (mg/min/mg microsomal protein),
Cprot is concentration of microsomal protein (mg/g tissue) and
Ftiss refers to the fractional volume of the metabolizing tissue (e.g., g liver/kg body weight)
From the in vivo Vmax given in the PLoS Comp Bio reference,I would like to calculate the in vitro Vmax for which I need the values of quantities Cprot and Ftiss.
In page 250,of the ref:https://www.crcpress.com/Principles-and-Methods-of-Toxicology-Fifth-Edition/Hayes/p/book/9781420005424
The Tissue volume (fraction of BW) for liver is given as 0.026.Where ,BW is Body weight of human: 70kgs
Would it be appropriate to use this value for Ftiss in the above equation.
Any suggestions on how to find the Cprot value for a given protein?I wish to obtain the Cprot values for all the enzymes present in glycolysis pathway.
I would be grateful for any help you can offer.