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Topic: TLC and Fluorescence  (Read 1010 times)

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Offline Hachiko

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TLC and Fluorescence
« on: June 16, 2019, 02:40:30 AM »
Could we detect an organic compound by TLC with UV lamp if it is not fluorescent?
« Last Edit: June 16, 2019, 03:12:39 AM by awesomesynthesis »

Offline AWK

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Re: TLC and Fluorescence
« Reply #1 on: June 16, 2019, 02:59:29 AM »
You may use many visualizing agents.
« Last Edit: June 16, 2019, 03:13:35 AM by AWK »
AWK

Offline Hachiko

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Re: TLC and Fluorescence
« Reply #2 on: June 16, 2019, 03:14:15 AM »
Sorry for my unclear question, I mean using UV lamp to detect non-fluorescent compound. So there is no way for UV lamp to detect this kind of compound?
This compound contains some of aromatic rings, could you recommend appropriate visualizing agent for this?

Offline AWK

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Re: TLC and Fluorescence
« Reply #3 on: June 16, 2019, 03:27:41 AM »
Some chromatographed compounds quench the indicator fluorescence and in this way become visible.
AWK

Offline Hachiko

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Re: TLC and Fluorescence
« Reply #4 on: June 16, 2019, 03:44:36 AM »
Thank you for your advice, but could you give some detail methods? I know about chemical stain but it's efficient used for some oxidizable groups or unsaturated bonds.

Offline AWK

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Re: TLC and Fluorescence
« Reply #5 on: June 16, 2019, 03:53:39 AM »
AWK

Offline Hachiko

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Re: TLC and Fluorescence
« Reply #6 on: June 16, 2019, 05:05:46 AM »
Thank you so much.

Offline Babcock_Hall

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Re: TLC and Fluorescence
« Reply #7 on: June 16, 2019, 12:52:54 PM »
Compounds with aromatic rings quenched fluorescence in my experience, but perhaps there are exceptions.  If you have other functional groups, perhaps we could help select a stain.

Offline Hachiko

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Re: TLC and Fluorescence
« Reply #8 on: June 17, 2019, 01:30:00 AM »
Sorry, I may get misunderstanding about mechanism of TLC monitor under UV light. So a non-fluorescent compound is also visible as a dark spot under UV light if it absorbs the UV light, is it? And a fluorescent compound is visible as a colored spot depending on the wavelength of fluorescence under UV light.

Offline rolnor

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Re: TLC and Fluorescence
« Reply #9 on: June 17, 2019, 06:30:52 AM »
Sorry, I may get misunderstanding about mechanism of TLC monitor under UV light. So a non-fluorescent compound is also visible as a dark spot under UV light if it absorbs the UV light, is it? And a fluorescent compound is visible as a colored spot depending on the wavelength of fluorescence under UV light.

Yes

Offline Hachiko

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Re: TLC and Fluorescence
« Reply #10 on: June 19, 2019, 03:28:30 AM »
Thank you all.

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