October 15, 2019, 02:38:12 AM
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Topic: The temperature of EDC/NHS experiment  (Read 203 times)

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Offline Mater1991

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The temperature of EDC/NHS experiment
« on: July 12, 2019, 05:50:29 AM »
Hello everyone:
I have some graphene oxide(GO) with  carboxylic acid functional groups that I wanted to couple to aminated graphene oxide(AGO).In order to activate carboxylic acids of graphene oxide that I put EDC/NHS to the solution.I have the question that how high the temperature of experiment EDC/NHS will fail,I want to get a fast and feasible reaction rate.
So,what's your temperature in EDC/NHS experiment,I wanted to get your opinions first. Any advice appreciated!

Some more info:
Solvent:DMF
Now the experimental temperature is 185℉/85℃

Offline wildfyr

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Re: The temperature of EDC/NHS experiment
« Reply #1 on: July 12, 2019, 09:10:39 AM »
I've always done it at room temperature overnight. I feel like the only time I've seen raised temps for it was to better solvate one of the reagents.

How did it go at 85°C?

Offline hollytara

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Re: The temperature of EDC/NHS experiment
« Reply #2 on: July 12, 2019, 10:11:57 AM »
My experience is at room temperature also - and usually in water.  Can you use a different coupling agent in DMF?

Offline wildfyr

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Re: The temperature of EDC/NHS experiment
« Reply #3 on: July 12, 2019, 11:30:21 AM »
Do it in acetonitrile. The DCU byproduct is poorly soluble in it, so the workup is easier.

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