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Topic: Antibody conjugation to COOH functionalized nanoparticles  (Read 1942 times)

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Offline Biotech78

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Antibody conjugation to COOH functionalized nanoparticles
« on: September 17, 2019, 05:57:13 AM »
Hi

Can anybody recommend a good paper which I can follow for EDC/NHS coupling of Ab to -COOH-NP. I know there are many out there but all the papers I see use huge quantities of Ab which I don't think I can afford when using a commercially available Ab.
Just haven't been able to get my head around what should be the ratio of NP and Ab

Offline wildfyr

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Re: Antibody conjugation to COOH functionalized nanoparticles
« Reply #1 on: September 18, 2019, 07:13:50 PM »
Try to work out the number of active sites on each nanoparticle. That should get you within an order of magnitude or two of what you need

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