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Topic: I need help about a spectrophotometry problem (for an exam)  (Read 1029 times)

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Offline Emster

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I need help about a spectrophotometry problem (for an exam)
« on: September 16, 2019, 02:20:35 PM »
I am lost for this one...

I have standards from phosphomolybdic acid that vary from 3 to 30 micrograms per cm cubic. I have the following information:
Slope = 0.425
Intercept = 0.015
Correl = 0.9995
Next, I have sample of 10ml of sea water that I need to analyze. The sample has been treated with the reagents and diluted to 25 ml. The absorbance of the sample is A=0.395 at a wavelenght of 600nm (1 cm wall). I need to find the concentration of the phosphates in the sample.

Any help would be appretiated. P.S. I don't have any more info, and I am doing this on paper, not in excel.

Offline chenbeier

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Re: I need help about a spectrophotometry problem (for an exam)
« Reply #1 on: September 16, 2019, 02:50:29 PM »
Do you know the Lambert-Beer Law.

You have standards and the parameters of the curve, so the Absorbance can be calculated.  For the analysis you calculate back from Absorbance to concentration

Offline Babcock_Hall

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Re: I need help about a spectrophotometry problem (for an exam)
« Reply #2 on: September 16, 2019, 03:52:03 PM »
Whenever I do dilution calculations, I draw a picture to help apply the dilution formula correctly.  I also use units in a consistent manner.  Many students run their ships aground in this calculation because of errors such as these.

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