December 14, 2019, 05:57:11 AM
Forum Rules: Read This Before Posting


Topic: Antibody conjugation to COOH functionalized nanoparticles  (Read 475 times)

0 Members and 1 Guest are viewing this topic.

Offline Biotech78

  • New Member
  • **
  • Posts: 8
  • Mole Snacks: +0/-0
Antibody conjugation to COOH functionalized nanoparticles
« on: September 17, 2019, 05:57:13 AM »
Hi

Can anybody recommend a good paper which I can follow for EDC/NHS coupling of Ab to -COOH-NP. I know there are many out there but all the papers I see use huge quantities of Ab which I don't think I can afford when using a commercially available Ab.
Just haven't been able to get my head around what should be the ratio of NP and Ab

Offline wildfyr

  • Global Moderator
  • Sr. Member
  • ***
  • Posts: 1376
  • Mole Snacks: +149/-9
Re: Antibody conjugation to COOH functionalized nanoparticles
« Reply #1 on: September 18, 2019, 07:13:50 PM »
Try to work out the number of active sites on each nanoparticle. That should get you within an order of magnitude or two of what you need

Sponsored Links