hey, ive been given the below problem in an assignment and have no idea how to solve it. Please help.
Prblem: A solution of iodide ions gave a fluorescence reading of 62%T. A 10 ml aliquot of a 0.2 mg/L iodide standard was added to 5ml of the solution. What is the concentration of iodide in the solution if the fluorescence intensity of the spiked solution was 84%T?
i know i must use the formula
where F= fluorescence
K= Fraction of emitted radiation being
collected, usually 90 degrees
O= fraction of species in sample which
deactivates to yield the measured emission.
b= light path
E= molar extinction coefficient
any help would be great.