December 14, 2019, 06:56:12 AM
Forum Rules: Read This Before Posting


Topic: How to quantify the amount of a specific protein in a sample of cells?  (Read 293 times)

0 Members and 1 Guest are viewing this topic.

NuzakFarnas

  • Guest
I'm interested in techniques that would allow you to quantify the amount of a specific protein in a cell culture, resulting in the weight (amount) of the protein in each culture sample. I'm aware of ChIP, affinity chromatography, and western blots, but not unaware of a technique to get the total quantity of the protein produced by a cell culture.


Offline Yggdrasil

  • Retired Staff
  • Sr. Member
  • *
  • Posts: 3207
  • Mole Snacks: +482/-21
  • Gender: Male
  • Physical Biochemist
Re: How to quantify the amount of a specific protein in a sample of cells?
« Reply #1 on: November 03, 2019, 11:54:23 AM »
One way would to be to perform a Western blot alongside a standard curve produced from known amounts of the purified protein.

Sponsored Links