The absorbance A of a solution is defined as:
In which I0 is the incident light intensity, and I is the transmitted light intensity. The absorbace is related to the molar absorption coefficient(extinction coefficient) ε (in M-1 cm-1), concentration c (in M) and path length l (in cm) by
The absorption coefficient of myoglobin at 580nm is 15,000 M-1 cm-1. What is the absorbance of a 1mg/ml solution across a 1cm light path? What percentage of the incident light is transmitted by this solution?
The first step that comes to mind is to convert mg/ml to moles/ml (since the absorption coefficient is in units of M-1 cm-1), but I'm not sure how to do that! Does anyone have any ideas on how to solve this problem?