Hello everybody, my name is Emilio and I'm a Ph.D. student in immunological oncology.
I've just been struggling with a cell culture which needs pure tetracycline for the selection of transfected cells.
I bought pure tetracycline hydrochloride and I do need the pure tetracycline. Given that my molecule is freely soluble in water, soluble in methanol and ethanol but is insoluble in ether and hydrocarbons, do you have some advice to get pure tetracycline?
Thanks everybody in advance for reading and answering, eventually.