April 03, 2020, 08:44:22 PM
Forum Rules: Read This Before Posting


Topic: Centrifuge question!  (Read 115 times)

0 Members and 1 Guest are viewing this topic.

Offline twertee

  • Very New Member
  • *
  • Posts: 1
  • Mole Snacks: +0/-0
Centrifuge question!
« on: March 26, 2020, 04:59:00 PM »
Hello all,

Quick centrifuge question for those of you experienced with benchwork that I have not been able to find addressed elsewhere. I'm doing a polar lipid analysis, the protocol says to use a biphasic mixture of hexane:methanol 1:1. I will then be centrifuging 5 mL of each within a 15 mL glass centrifuge tube 3000 rpm x 5 minutes. When centrifuging hexane, would it be appropriate to use a glass centrifuge tube that is capped or without a cap? I concerned about expansion from hexane but I am unsure if it would be good practice to use hexane in an uncapped centrifuge tube due to its volatility.

Thanks!

Offline chenbeier

  • Full Member
  • ****
  • Posts: 873
  • Mole Snacks: +71/-17
  • Gender: Male
Re: Centrifuge question!
« Reply #1 on: March 26, 2020, 05:29:29 PM »
You should cap the tube, because hexane can fume similar petrolgas . The electrical parts of the centrifuge can ignite it. Its s safety issue. For methanole the same.

Offline Babcock_Hall

  • Chemist
  • Sr. Member
  • *
  • Posts: 4014
  • Mole Snacks: +249/-16
Re: Centrifuge question!
« Reply #2 on: March 26, 2020, 08:08:30 PM »
I am not familiar with this particular application.  However, I would like to ask a question.  Are these Corex centrifuge tubes?

Sponsored Links