My compound is low soluble so I need much polar solvent to dissolve it, when I runned column, I got the mixing. I think the reason is that my solution had low concentration and polar solvent make my sample all run together. I can't use dry loading because my compound can be soluble in solvent system. I try recrystallization but it's not good.
Could you share your opinion and experience when you handle with low solubility compound in column chromatography?