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Centrifuge question!

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twertee:
Hello all,

Quick centrifuge question for those of you experienced with benchwork that I have not been able to find addressed elsewhere. I'm doing a polar lipid analysis, the protocol says to use a biphasic mixture of hexane:methanol 1:1. I will then be centrifuging 5 mL of each within a 15 mL glass centrifuge tube 3000 rpm x 5 minutes. When centrifuging hexane, would it be appropriate to use a glass centrifuge tube that is capped or without a cap? I concerned about expansion from hexane but I am unsure if it would be good practice to use hexane in an uncapped centrifuge tube due to its volatility.

Thanks!

chenbeier:
You should cap the tube, because hexane can fume similar petrolgas . The electrical parts of the centrifuge can ignite it. Its s safety issue. For methanole the same.

Babcock_Hall:
I am not familiar with this particular application.  However, I would like to ask a question.  Are these Corex centrifuge tubes?

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