June 07, 2020, 02:33:32 AM
Forum Rules: Read This Before Posting


Topic: HPLC: Can I read peak resolution from two different chromatograms?  (Read 186 times)

0 Members and 1 Guest are viewing this topic.

Offline txxplc110

  • New Member
  • **
  • Posts: 3
  • Mole Snacks: +0/-0
Hello!
I have a question about HPLC chromatograms. I got two chromatograms, each one with different signal/wavelength (290nm and 320nm) and I got two peaks of compounds that I wanted to analyze, but the peaks are not on the same chromatogram. My question is, can I read peak resolution of two peaks from two different chromatograms, or not? It doesn’t make any sense for me, but it's in my instruction. I can’t find any information about that. I would be most grateful if you would look into this matter.

Offline shchavel

  • Regular Member
  • ***
  • Posts: 23
  • Mole Snacks: +0/-0
Re: HPLC: Can I read peak resolution from two different chromatograms?
« Reply #1 on: May 09, 2020, 03:23:45 AM »
Hello!
I am not a HPLS specialist, but i think, that it's uncorrect. You must get this peaks in 1 expirement/chromatogramm to correct compare.

Offline txxplc110

  • New Member
  • **
  • Posts: 3
  • Mole Snacks: +0/-0
Re: HPLC: Can I read peak resolution from two different chromatograms?
« Reply #2 on: May 09, 2020, 02:51:07 PM »
Hello!
I am not a HPLS specialist, but i think, that it's uncorrect. You must get this peaks in 1 expirement/chromatogramm to correct compare.
It turned out to be correct. It was in the same injection but at a different wavelength and an expert told me that I can calculate it. :)

Sponsored Links