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Offline samra

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need help urgent 1 day left
« on: August 10, 2020, 04:07:28 PM »
You are given a mixture of quercetin and gingerol that need to be separated. Unfortunately, these two compounds have very similar boiling points, molecular weights, and polarities. Describe a method that should provide a baseline separation of these two compounds and explain why it should work. Give details of the type of column, detector, and any other major equipment or procedures necessary to complete your separation. Using your proposed method, which compound should elute first and why?   

Offline Borek

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Re: need help urgent 1 day left
« Reply #1 on: August 10, 2020, 04:21:19 PM »
Please read the forum rules that you agreed to during registration.

I am deleting all your other spammy posts. We will be happy to help you if you ask your questions in a correct manner.
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Offline Enthalpy

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Re: need help urgent 1 day left
« Reply #2 on: August 12, 2020, 07:50:02 AM »
The question contains the demanded answer, with "column" and "elute".

The melting points are very far from an other, this might have been an alternative.

Offline rolnor

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Re: need help urgent 1 day left
« Reply #3 on: August 12, 2020, 09:07:18 AM »
I think they are very different in polarity/hydrophobisity, both a straight-phase or reversed-phase column would separate these two without any problem. Quercetine on straight-phase is EtOAc/MeOH 20:1 Gingerol is EtOAc/hexane 1:1 is my suggestion. You can use a gradient with these two mobile-phases.
« Last Edit: August 12, 2020, 09:48:28 AM by rolnor »

Offline wildfyr

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Re: need help urgent 1 day left
« Reply #4 on: August 12, 2020, 02:21:26 PM »
You could use a mobile phase that takes advantage of the ligation chemistry of the catechol moiety.

Offline Babcock_Hall

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Re: need help urgent 1 day left
« Reply #5 on: August 13, 2020, 12:50:01 PM »
Or a stationary phase that does.

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