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Protein assay - determining amount of protein from standard curve

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yogurtspoon:
mjc123: We plotted absorbances vs. the concentrations of the final samples (same amount of protein in 1.6 mL). My bad on the editing thing as well, I'm new on this forum and thought reddit etiquette applied.

Babcock_Hall: I'm honestly not sure. The most concentrated unknown should just about match my most concentrated standard, so wouldn't deviations be caused by my own imprecisions when I prepared the solutions?

Babcock_Hall:
If had an unknown protein solution and had no idea of what its concentration was, I might choose a dilution protocol for it that caused it to have a larger concentration than the highest standard.  For that matter I might just as easily pick a dilution factor that caused it to have a lower concentration than the lowest standard.

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