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Column chromatography
Jason09:
Hi there,
in my 4 years of studying med I've never come across something so complicated to understand..
How does one go about using (gradient: 0–40% ethyl acetate in hexanes to isolate sugars)
Do you first run the column starting from 0% ethyl acetate in hexanes and work your way up?
Babcock_Hall:
Yes, that is what I would infer. Can you think of a reason for using a gradient?
The manner of increasing the percentage of ethyl acetate might be different depending on the particulars. Are we talking about high performance liquid chromatography?
Jason09:
Yes, it will be a high performance liquid chromatography. Also, does running the column with 0% ethyl acetate in hexanes at first mean the whole column into the flask or partially?
Babcock_Hall:
It means that solvent from a reservoir of hexanes is pumped through the column at first. Then there is a mixer which gradually adds more and more ethyl acetate (from a separate reservoir) to the solvent that enters the column. Have you ever used high performance liquid chromatography previously?
Jason09:
Thanks for the replies Babcock that was actually a good explanation.
No unfortunately, first time.
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