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Can michaelis menton equation determine IC50

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distheho:
If I plot the % reduced (from uninhibited reaction) enzyme reaction rate vs inhibitor concentration, is the "Km" the IC50 for that specific enzyme concentration?

Babcock_Hall:
I am a little puzzled by your reference to the enzyme concentration.  Can you explain?

distheho:
It is my understanding that IC50 is based on enzyme concentration.

Babcock_Hall:
https://pubmed.ncbi.nlm.nih.gov/4202581/
There is a 1973 paper by Cheng and Prusoff that discusses the meanings of IC50.  With respect to the Michaelis-Menten equation, KM does not depend on enzyme concentration.  Just glancing at the paper, IC50 values do depend on substrate concentration.

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