I am so sorry for such a newbie question. I am a retired person trying to learn chemistry during the pandemic, and I need some help. I am trying to figure out how to do a basic calculation for chromatography.
If I take a sample of something/anything, that weighs 5 grams, and I put it in a solvent, lets say it is 50 ml. I pipette off 2 ml of that and inject it into a liquid chromatograph. Obviously I would need to have already done a calibration on the liquid chromatograph, and I would have integrated peaks along the way to get that calibration. But if I run that sample, and I get a peak that I integrate - how do I calculate the concentration in that 5 gram sample?
I get that I have the area of the peak, and I compare it to the calibration peaks - but how do you account for the size of the sample, the volume of the solvent, and use that to figure the concentration?
Thank you for anyone who is willing to take the time to explain!