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Topic: Extremely small absorbance differences?  (Read 698 times)

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Offline scienceaddiction1

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Extremely small absorbance differences?
« on: May 26, 2021, 04:05:52 PM »
I am trying to measure the absorbance of samples which have varying levels of emulsified bisphenol-a in them with a spectrophotometer. I'm using the absorbance vs concentration setting and am also using a sample that does not have bisphenol-a as a calibrant.

However, when I go to measure the samples (same quantity and procedure) I get extremely small absorbance level differences (.001-.003) which are within the margin of error. I've tried this at 230 and 270nm wavelengths, which are said to be optimal for bisphenol-a detection.

The amount of BPA that should be in these samples is non-negligible and is high compared to real world scenarios.

What could I be doing wrong?

Thank you for any that can be offered!

Offline Orcio_87

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Re: Extremely small absorbance differences?
« Reply #1 on: May 26, 2021, 04:27:09 PM »
Does the small absorbance relates only to samples of smallest concentration or also every other sample ?

Quote
The amount of BPA that should be in these samples is non-negligible and is high compared to real world scenarios
Are these samples a field samples (taken to measure concentration of bis-phenol) or are they calibration samples ?

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