Chemistry Forums for Students > Analytical Chemistry Forum

derivitisation and LC/MS of vegetable oils

<< < (2/2)

Steville:
Hi all,

Thanks for the excellent responses!

I'll try the GC route and see if I can get a standard sample with a variety of esters.
I'll keep you all posted and tell you if I overload the column!

Stephen

wildfyr:
Definitely a job for GCMS.

One great trick is that if you dissolve all your crap in MeOH and add a tiny amount of sulfuric acid and let it stir, everything will come out methyl ester. Its dead easy.

ablake:
We use butyl esterification for medium chain fatty acids on GCMS. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224454
Same method, just leave out the silylation step. Just make sure to include a good post run oven temperature ramp to purge out everything and prevent sample carryover.

Also, I would suggest getting a deuterated internal standard of a short, medium, and long chain FA at the minimum. Don't get standards that are already esterified because then it wont account for if the esterification step went to completion on your unknowns/samples.

Navigation

[0] Message Index

[*] Previous page