October 17, 2021, 10:15:19 PM
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Topic: chromatography resolution and retention  (Read 93 times)

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Offline xshadow

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chromatography resolution and retention
« on: October 09, 2021, 07:00:56 AM »
Hi, I have some ""doubts"" about the chemical-phisical meaning of the "retention" contribute that appears in resolution expression:

RAB= efficiency + selectivity + KB / (1+ KB)

Where A and B are two solutes (and B elutes last)
The  KB / (1+ KB) is the "retention"

Now looking at its expression I can see that:

a) when KB >> 1 the retention is KB/KB = 1
b) from that formula  it seems that retention goes from 0 to 1.
c) ...so in order to increase retention (and therefore resolution) I need to increase  KB

is it correct?

Now why should a higher retention time (expressed though a higher KB  increase resolution???
What is the analitical chemistry "reason" ?!


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