Hi, I have some ""doubts"" about the chemical-phisical meaning of the "retention" contribute that appears in resolution expression:
R
AB= efficiency + selectivity + K
B / (1+ K
B)
Where A and B are two solutes (and B elutes last)
The K
B / (1+ K
B) is the "retention"
Now looking at its expression I can see that:
a) when K
B >> 1 the retention is K
B/K
B = 1
b) from that formula it seems that retention goes from 0 to 1.
c) ...so in order to increase retention (and therefore resolution) I need to increase K
Bis it correct?
Now why should a higher retention time (expressed though a higher K
B increase resolution???
What is the analitical chemistry "reason" ?!
THANKS