March 29, 2024, 07:13:57 AM
Forum Rules: Read This Before Posting


Topic: chromatography resolution and retention  (Read 1075 times)

0 Members and 1 Guest are viewing this topic.

Offline xshadow

  • Full Member
  • ****
  • Posts: 427
  • Mole Snacks: +1/-0
chromatography resolution and retention
« on: October 09, 2021, 07:00:56 AM »
Hi, I have some ""doubts"" about the chemical-phisical meaning of the "retention" contribute that appears in resolution expression:

RAB= efficiency + selectivity + KB / (1+ KB)

Where A and B are two solutes (and B elutes last)
The  KB / (1+ KB) is the "retention"

Now looking at its expression I can see that:

a) when KB >> 1 the retention is KB/KB = 1
b) from that formula  it seems that retention goes from 0 to 1.
c) ...so in order to increase retention (and therefore resolution) I need to increase  KB

is it correct?

Now why should a higher retention time (expressed though a higher KB  increase resolution???
What is the analitical chemistry "reason" ?!

THANKS :)

Sponsored Links