Just recently, I have been giving some serious thought into culturing some bacteria, pseudomonas in particular, to harvest a particular enzyme from them.
Pseudomonas I know are commonly found growing on old bath sponges/loofahs etc. and are known for causing problems when scratched into the skin by brushes or loofahs, has anyone got any details of how to identify pseudomonas bacteria, and pictures they could upload?
Also, I am very inexperienced with biological cultures, could some kind preson perhaps guide me through a good process to grow these bacteria, I have no fancy equipment, but making up nutrient solutions with multivitamin pills, minerals etc. and sterililizing solutions is no problem.
I aim for quite a large colony, so as to extract the enzyme out of the bacteria,
if possible, I want to be able to use a two-liter coke bottle as a growing chamber, to ensure a large crop of bacteria, petri dishes are too small by far, and I only have one (I "liberated" it from school about three years ago