January 17, 2022, 08:28:44 PM
Forum Rules: Read This Before Posting


Topic: Zip tips for cleaning up tryptic digests for LCMS  (Read 129 times)

0 Members and 1 Guest are viewing this topic.

Offline Babcock_Hall

  • Chemist
  • Sr. Member
  • *
  • Posts: 5068
  • Mole Snacks: +294/-22
Zip tips for cleaning up tryptic digests for LCMS
« on: December 03, 2021, 02:07:24 PM »
Our interest is in LC/MS of the peptides, and we plan to work in water/acetonitrile/formic acid.  We found a number of protocols for Zip Tips (ours have C-18 packing) on-line, and we are focusing on those protocols that specify formic acid (not TFA).  I can post links to anything that we found.  (1) Are Zip Tips necessary?  (2) Given their capacity, does one obtain enough for material for one or for several LC/MS runs?  (3) Are Zip Tips reusable? (4) Does anyone have a particular paper or protocol that is particularly informative?  Thank you very much.

Sponsored Links