March 29, 2024, 08:08:29 AM
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Topic: Zip tips for cleaning up tryptic digests for LCMS  (Read 1271 times)

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Offline Babcock_Hall

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Zip tips for cleaning up tryptic digests for LCMS
« on: December 03, 2021, 02:07:24 PM »
Our interest is in LC/MS of the peptides, and we plan to work in water/acetonitrile/formic acid.  We found a number of protocols for Zip Tips (ours have C-18 packing) on-line, and we are focusing on those protocols that specify formic acid (not TFA).  I can post links to anything that we found.  (1) Are Zip Tips necessary?  (2) Given their capacity, does one obtain enough for material for one or for several LC/MS runs?  (3) Are Zip Tips reusable? (4) Does anyone have a particular paper or protocol that is particularly informative?  Thank you very much.

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