December 04, 2022, 06:42:03 AM
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Topic: Analyzing protein sample via UV-Vis  (Read 1650 times)

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Soulja

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Analyzing protein sample via UV-Vis
« on: December 21, 2021, 06:42:14 PM »
Hey all,

I am working on a project involving an extraction of an allergen protein. I was able to extract it from the raw source, and it is currently in a freezer. My work requires me to analyze it via several methods of analysis. I am having trouble running my protein via UV-Vis; the main problem being that my protein just doesn't seem to be soluble in any solvents I use. So far I've tried acetone and methanol (which was used in the extraction process), but i've had no luck with sonication and stirring. Does anyone have any potential suggestions?

THanks.

Offline jeffmoonchop

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Re: Analyzing protein sample via UV-Vis
« Reply #1 on: December 22, 2021, 03:04:32 PM »
How about water?

Offline Orcio_87

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Re: Analyzing protein sample via UV-Vis
« Reply #2 on: December 22, 2021, 04:23:15 PM »
Water may not help if the methanol denatured the protein.

Offline Corribus

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Re: Analyzing protein sample via UV-Vis
« Reply #3 on: December 23, 2021, 01:40:17 PM »
What are you hoping that UV-Vis will tell you?
What men are poets who can speak of Jupiter if he were like a man, but if he is an immense spinning sphere of methane and ammonia must be silent?  - Richard P. Feynman

Soulja

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Analyzing protein sample via UV-Vis
« Reply #4 on: December 25, 2021, 03:44:42 PM »
Hey all,

I am working on a project involving an extraction of an allergen protein. I was able to extract it from the raw source, and it is currently in a freezer. My work requires me to analyze it via several methods of analysis. I am having trouble running my protein via UV-Vis; the main problem being that my protein just doesn't seem to be soluble in any solvents I use. So far I've tried acetone and methanol (which was used in the extraction process), but i've had no luck with sonication and stirring. Does anyone have any potential suggestions?

THanks.

Offline billnotgatez

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Re: Analyzing protein sample via UV-Vis
« Reply #5 on: December 25, 2021, 04:54:36 PM »
we do not double post here
check forum rules
I merged past and present posts
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« Last Edit: December 25, 2021, 05:10:24 PM by billnotgatez »

Offline Babcock_Hall

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Re: Analyzing protein sample via UV-Vis
« Reply #6 on: December 30, 2021, 10:56:15 AM »
Buffered water is the first thing that I can think of.  Acetone is likely to precipitate a protein.

Offline morphism

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Re: Analyzing protein sample via UV-Vis
« Reply #7 on: January 07, 2022, 08:49:46 AM »
Hi! What kind of allergen protein?

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