July 07, 2022, 03:30:50 AM
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Topic: How to choose the detection wavelength for HILIC ?  (Read 280 times)

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Offline PharmaStud.

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How to choose the detection wavelength for HILIC ?
« on: April 20, 2022, 05:49:10 PM »
Hello,

I have to analyze 5-fluorouracil and its impurities (5-methoxyuracil, barbituric acid and urea) by the HILIC method with a UV detector. But what I don't understand is how to choose the detection wavelength, 5-Fluorouracil, 5-Methoxyuracil and Barbituric acid absorb at around 265 nm. Urea is a molecule that is analyzed at 200nm because it does not absorb on higher wavelengths. As I have to analyze them by the same method, I don't understand which wavelength should I choose ? Should I choose the urea wavelength or choose a wavelength that is between 200 and 265 nm ?

Thanks,

Offline Babcock_Hall

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Re: How to choose the detection wavelength for HILIC ?
« Reply #1 on: April 21, 2022, 08:26:24 AM »
Some detectors are able to monitor more than one wavelength.  Is that the case for your instrument?

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