Hello! I'm a Pharmacy student and I've been following an Analitical Chemistry course during this semester.
I was messing around with the European Pharmacopoeia, trying to read and understand a few monographs.
I stumbled upon Atenolol and I was wondering what type of cromatography you're supposed to perform. Is it an ion-pairing crompatography?
Here's the text:
Related substances. Liquid chromatography (2.2.29).
Test solution. Dissolve 50 mg of the substance to be examined
in 20 mL of the mobile phase and dilute to 25.0 mL with the
Reference solution (a). Dissolve 2 mg of atenolol for system
suitability CRS (containing impurities B, F, G, I and J) in 1 mL
of the mobile phase.
Reference solution (b). Dilute 1.0 mL of the test solution to
100.0 mL with the mobile phase. Dilute 1.0 mL of this solution
to 10.0 mL with the mobile phase.
– size : l = 0.125 m, Ø = 4.0 mm;
– stationary phase : end-capped octadecylsilyl silica gel for
chromatography R (5 μm).
Mobile phase : dissolve 1.0 g of sodium octanesulfonate R and
0.4 g of tetrabutylammonium hydrogen sulfate R in 1000 mL
of a mixture of 20 volumes of tetrahydrofuran R, 180 volumes
of methanol R2 and 800 volumes of a 3.4 g/L solution of
potassium dihydrogen phosphate R; adjust the apparent pH
to 3.0 with phosphoric acid R.
Flow rate : 0.6 mL/min.
Detection: spectrophotometer at 226 nm.