August 10, 2022, 07:46:38 AM
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Topic: reverse phase chromatography of polar compounds  (Read 98 times)

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Offline bashert

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reverse phase chromatography of polar compounds
« on: August 01, 2022, 12:55:08 PM »
I’m working on the separation of two polar compounds. I’ve been reading up on reversed phase chromatography and just trying to understand one aspect. In an online guide from Waters I read (https://www.waters.com/webassets/cms/library/docs/wa20756.pdf):

“To retain polar compounds on this non polar surface we reduce the amount of organic in the mobile phase (i.e., 100% water).”

I’m confused because by doing that you’re making the mobile phase even more polar….wouldn’t that make it a better solvent for a polar molecule!? Then why would that cause a polar molecule to bind the nonpolar stationary phase?? What am I missing?

Thanks!

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