December 07, 2022, 01:46:58 AM
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Topic: Thin Layer Chromatography - No Standard  (Read 237 times)

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Offline trichocereus

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Thin Layer Chromatography - No Standard
« on: November 19, 2022, 01:58:44 PM »
Hi, I'm quantifying the amount of alkaloids present in a San Pedro cacti (Echinopsis pachanoi (formerly Trichocereus pachanoi)). I am not trying to look at just one alkaloid but mescaline (illegal to extract on it it's own) is the primary one. I decided to do Thin Layer Chromatography as an attempt to quantify them. I've seen other studies say they used Mescaline Sulphate as their standard, something I obviously again can't get my hands on because it's illegal. What should I do? I don't know another way to quantify the alkaloids present and I can't get my hands on the standard; what else can I do? Can I use something else as the standard?

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