Hello, I'm currently trying to characterize a macrocycle by NMR, but I'm having difficulty executing it.
My basic articles do the analysis using methanol-d4 or deuterium oxide.
However, in my tests, the molecule has extreme difficulty being solubilized in these solvents, causing the solution to precipitate with just a few milligrams added.
I filter the solutions when they present precipitation, but the NMR cannot capture the molecule, even when I use high frequencies.
I know that the molecule easily solubilizes in NaOH.
I'm thinking of preparing NaOH + D2O and doing the NMR later, could this solution cause any possible interference in the analysis?
I don't know how to proceed