March 21, 2023, 03:48:07 AM
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Topic: Can you calibrate micropipets for for all liquids  (Read 164 times)

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Offline PSbP2VP/NMP+PtCl62-

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Can you calibrate micropipets for for all liquids
« on: March 12, 2023, 06:19:12 PM »
If you calibrate a micropipet with water, is it effectively calibrated for all other liquids (e.g. DMSO, ether, polymer solutions)? If not, how does one go about making trustworthy measurements with micropipets in an atmosphere where they're used by several researchers to measure various liquid solutions.

Side note: I have a general mistrust of pipets as volumetric measurement tools (PTSD from having to repeat weeks of ICP-MS measurements in gradschool) and never really use them for measurements, so I've never bothered to have my micropipet set calibrated (the balance gets biannual professional calibration and business class treatment in the lab).

Offline DrCMS

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Re: Can you calibrate micropipets for for all liquids
« Reply #1 on: March 13, 2023, 06:20:00 AM »
If you calibrate a micropipet with water, is it effectively calibrated for all other liquids (e.g. DMSO, ether, polymer solutions)?

No it is not.  If have calibrated with a liquid with a particular density/viscosity/surface tension and then use a liquid that does not have exactly the same density and viscosity and surface tension it will not dispense the same calibrated volume.

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