March 29, 2024, 06:35:36 AM
Forum Rules: Read This Before Posting


Topic: Reference Cubette  (Read 747 times)

0 Members and 1 Guest are viewing this topic.

Offline kobold

  • Very New Member
  • *
  • Posts: 1
  • Mole Snacks: +0/-0
Reference Cubette
« on: March 23, 2023, 11:16:51 AM »
Hi!

I got a double beam spectrophotometer. I am using a method of phosphurus determination using Ammonium Hepta-Molybdate at 880 nm.

My question is simple: is it correct if I fill the reference cubette with distilled water (because it is the solvent I use)?
Or it would be better to use a blank (everything I use to make the samples without the sample) ?

I am kinda leaning to the opinion that it does not matter which I use but I want your opinion!

Thank you all for your attention!

Offline Hunter2

  • Sr. Member
  • *****
  • Posts: 2155
  • Mole Snacks: +162/-47
  • Gender: Male
  • Vena Lausa moris pax drux bis totis
Re: Reference Cubette
« Reply #1 on: March 23, 2023, 12:25:46 PM »
The blank should be done with the same matrix. So the best is to make one with  everything in without the phosphate.
But you can compare a measurement with distilled water, how much is difference to Zero.

Sponsored Links