May 31, 2023, 06:51:52 AM
Forum Rules: Read This Before Posting


Topic: Michaelis-menten  (Read 285 times)

0 Members and 1 Guest are viewing this topic.

Offline Iridonia

  • Very New Member
  • *
  • Posts: 1
  • Mole Snacks: +0/-0
Michaelis-menten
« on: May 15, 2023, 04:22:09 AM »
Hey.

I'm really struggling with this one. In regards to which steps I've taken to solve it on my own, I got nothing but some pathetic attempts to isolate [ S] or play around with the formula. I'm spectacularly bad at this type of chemistry. I've read up on it but I still don't get it.
I'll try to translate it to the best of my ability.

The link between the substrate concentration [ S] and the initial velocity V0 is given by the following equation:

V0 = Vmax*[ S]/Km+[ S]

Indicate the amount of [ S] (in relation to Km) in which V0 = 66,67% of the maximal velocity.

Thank you

Offline Borek

  • Mr. pH
  • Administrator
  • Deity Member
  • *
  • Posts: 27355
  • Mole Snacks: +1776/-408
  • Gender: Male
  • I am known to be occasionally wrong.
    • Chembuddy
Re: Michaelis-menten
« Reply #1 on: May 15, 2023, 08:07:13 AM »
From the sound of it yours is not a problem with chemistry, but with algebra.

Note: shorthand that you used to enter the formula is incorrect, as it suggests second [itex]\left[S\right][/itex] is not part of the denominator. Adding parentheses will make the notation unambiguous.

Start by multiplying both sides by [itex](K_m + \left[S\right])[/itex].
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info, pH-meter.info

Offline Babcock_Hall

  • Chemist
  • Sr. Member
  • *
  • Posts: 5416
  • Mole Snacks: +307/-22
Re: Michaelis-menten
« Reply #2 on: May 15, 2023, 10:10:26 AM »
@OP,

Borek made a good point about notation.  An alternative way to perform the algebra is to divide both sides by Vmax.

Offline Borek

  • Mr. pH
  • Administrator
  • Deity Member
  • *
  • Posts: 27355
  • Mole Snacks: +1776/-408
  • Gender: Male
  • I am known to be occasionally wrong.
    • Chembuddy
Re: Michaelis-menten
« Reply #3 on: May 15, 2023, 04:49:39 PM »
An alternative way to perform the algebra is to divide both sides by Vmax.

While it is definitely a necessary step it won't help in solving for [itex]\left[S\right][/itex], which is probably the most challenging part of the manipulation.
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info, pH-meter.info

Sponsored Links