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Topic: problems dissolving tetrazolium chloride granules  (Read 949 times)

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Offline JMule71

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problems dissolving tetrazolium chloride granules
« on: October 17, 2023, 04:13:27 PM »
Hello, I am hoping someone can help me as I think this is a very easy question for those with a background in chemistry. I am hoping to produce a 1% solution of 2,3,5-Triphenyl-2H-tetrazolium chloride (98%) for the purposes of staining seeds to test for viability. Available procedures suggest dissolving 1g into 100ml of water, using water, ethanol, methanol, acetone or DMSO as a solvent.
I have tried dissolving 1 gram into 100ml of distilled water and it didn't dissolve as there were many white crystals still present. I then tried dissolving the granules in ~10 ml of 96% Isopropyl alcohol and then added 90 ml warm distilled water with the same results, many white crystals present.
The chemical is from Thomas Scientific and information is as follows:
CAS#:298-96-4
Form: solid
Formula: C19H15CIN4
Formula Weight: 334.80
Sensitivity 1: Hygroscopic
Sensitivity 2: Light sensitive

Any help would be appreciated.

Offline Hunter2

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Re: problems dissolving tetrazolium chloride granules
« Reply #1 on: October 17, 2023, 06:42:56 PM »
The solubility  is approx. 50 g/l in water and 10 g/l in alcohol. Probably your product is impure. Try to add some drops of HCl.

Offline JMule71

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Re: problems dissolving tetrazolium chloride granules
« Reply #2 on: October 17, 2023, 06:48:41 PM »
Thank you, I will try this.

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