February 21, 2024, 02:22:45 PM
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Topic: HPLC- Determine the concentration of a compound in a sample  (Read 177 times)

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Offline Studenthelp2

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HPLC- Determine the concentration of a compound in a sample
« on: February 08, 2024, 04:33:37 PM »
Hello, I am a student (not chemistry) who needs help with a chemistry problem for an undergrad project. I carried out HPLC-DAD testing on a sample. I am trying to determine the concentration of a substance in a sample I purchased. This sample claims to have 20mg of compound in a 10ml vial. (This is Unlikely). Using 1mg of reference standard of compound (1024.18g/mol) I created 50ppm, 150ppm and 200ppm stock solutions and tan this in HPLC I got calibration curve for this (peak at 4 minutes) which is:

Y=37734X + 2E+ 06

I ran my samples of the unknown concentration in 1ml + 1ml water and then 2ml concentrations. The peak area I got for this was:

1ml + 1ml water = 12570265

2ml =18025927

I am looking for help to determine the concentration in Mg/ml of compound in my samples but am unsure of what to do next and could do with some *delete me*

Thanks in advance:) (I hope I explained this well and apologise for any confusion) 

Offline Borek

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Re: HPLC- Determine the concentration of a compound in a sample
« Reply #1 on: February 09, 2024, 02:55:26 AM »
First step: signal is what your calibration curve refers to as Y. Simple math - solve for X, plug Y, calculate.
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