May 29, 2024, 05:08:36 AM
Forum Rules: Read This Before Posting


Topic: Making a stock solution in DMSO  (Read 1829 times)

0 Members and 1 Guest are viewing this topic.

Offline hubbcut

  • New Member
  • **
  • Posts: 4
  • Mole Snacks: +0/-0
Making a stock solution in DMSO
« on: March 06, 2024, 09:48:35 AM »
Hi! What does it mean to make a 1M stock solution of Nile Red in DMSO? I've never worked with either reagent before and unsure of the calculations to make this. How do I go about it?

Offline Borek

  • Mr. pH
  • Administrator
  • Deity Member
  • *
  • Posts: 27689
  • Mole Snacks: +1803/-410
  • Gender: Male
  • I am known to be occasionally wrong.
    • Chembuddy
Re: Making a stock solution in DMSO
« Reply #1 on: March 06, 2024, 11:37:45 AM »
Don't get sidetracked by the substances involved, it is a just a solute and solvent. Concentration is number of moles of Nile Red per volume of the solution, nothing more fancy.
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info

Offline hubbcut

  • New Member
  • **
  • Posts: 4
  • Mole Snacks: +0/-0
Re: Making a stock solution in DMSO
« Reply #2 on: March 07, 2024, 04:52:10 AM »
Don't get sidetracked by the substances involved, it is a just a solute and solvent. Concentration is number of moles of Nile Red per volume of the solution, nothing more fancy.

Yes but I'm unsure how to start. I don't know how much DMSO I'm supposed to dissolve into water. The paper I'm using as a basis for my study only says to make a 1M stock solution of Nile Red, but doesn't say how much DMSO is needed in the final concentration.

Offline Hunter2

  • Sr. Member
  • *****
  • Posts: 2208
  • Mole Snacks: +176/-48
  • Gender: Male
  • Vena Lausa moris pax drux bis totis
Re: Making a stock solution in DMSO
« Reply #3 on: March 07, 2024, 05:07:30 AM »
I think there is no water involved. The stock solution uses DMSO as the solvent.

Offline Babcock_Hall

  • Chemist
  • Sr. Member
  • *
  • Posts: 5630
  • Mole Snacks: +324/-24
Re: Making a stock solution in DMSO
« Reply #4 on: March 07, 2024, 01:40:05 PM »
https://docs.aatbio.com/products/protocol/22190.pdf

I wonder whether you might mean a 1 mM stock solution in DMSO.  I am not sure what the solubility limit is.

Offline Hunter2

  • Sr. Member
  • *****
  • Posts: 2208
  • Mole Snacks: +176/-48
  • Gender: Male
  • Vena Lausa moris pax drux bis totis
Re: Making a stock solution in DMSO
« Reply #5 on: March 07, 2024, 03:44:21 PM »
In the TDS its clearly written to use  anhydrous DMSO.

Offline rjb

  • Full Member
  • ****
  • Posts: 124
  • Mole Snacks: +17/-0
Re: Making a stock solution in DMSO
« Reply #6 on: March 07, 2024, 07:00:16 PM »
https://docs.aatbio.com/products/protocol/22190.pdf

I wonder whether you might mean a 1 mM stock solution in DMSO.  I am not sure what the solubility limit is.

This takes me back! I think you're right... According to my old scribbled notes, it's soluble in DMSO up to about 10mM. With current prices a 1M solution would be quite an expensive undertaking!

R

Sponsored Links