[cxc621]

I have a trouble during operating our 3200 qtrap system that we just get it in second hand. I connected N2 on pressure 30 psi according to the manual that says the curtain gas should be turned on and flow should be as least 20 psi(see https://drive.google.com/file/d/1tbCTtXIVBXiDfI56okTk_f3OXy14WhN8/view?usp=sharing). After I switched on vacuum pump, open Analyst software and active 3200 qtrap by configuring synchronization trigger at active low, building acquisition method with MS scanning type in positive Q1 MS (Q1),with starting and stopping at 200Da and 700 Da at 2.5 second, building acquisition batch based on this method and submit it. I got error message that says "Could not submit the batch data; DABServer Error=0x80040400 Acquisition method(s) selected is inconsistent with the active Hardware Profile. Check the Hardware Profile”(see https://drive.google.com/file/d/1wxEkHAO9D6kDHX0uM2-uZ5h2sQ5-OdeQ/view?usp=sharing). At the same times, the status ready light (green) of 3200 qtrap was extinguished after flash in several minute (see https://drive.google.com/file/d/164drfB07jSgMdqkIJ4FUsC8zmpE82fqa/view?usp=sharing)(it shows what the manual says about the status ready light). Is it normal for this ready light extinguishing? Is any idea for solving this batch error?

[MOTOBALL]

Well, to begin with, you do not start pumping gas and turning the electronics on until the whole system is under high vacuum, i.e. the vacuum pumps (roughing pump and hv pumps ) have been on usually overnight.
1) pump down to around 1x 10 to -6 mm
2) electronics ON
3) Gas flows ON
4) set temperatures of source etc.
5) Allow 4 & 5 to stabilize
6) Infuse a solution of your tuning compound
7) Optimize the signal over your range of interest (m/z 200 -700)
Enter your method descriptor (scan m/z 200-700, generally 1 sec)

Let us know if this works for your system.

Regards,
MOTOBALL