December 11, 2024, 07:15:34 AM
Forum Rules: Read This Before Posting


Topic: problems dissolving tetrazolium chloride granules  (Read 1078 times)

0 Members and 1 Guest are viewing this topic.

cavebt

  • Guest
problems dissolving tetrazolium chloride granules
« on: November 06, 2024, 02:25:44 AM »
Hello, I am hoping someone can help me as I think this is a very easy question for those with a background in chemistry. I am hoping to produce a 1% solution of 2,3,5-Triphenyl-2H-tetrazolium chloride (98%) for the purposes of staining seeds to test for viability. Available procedures suggest dissolving 1g into 100ml of water, using water, ethanol, methanol, acetone or DMSO as a solvent.
I have tried dissolving 1 gram into 100ml of distilled water and it didn't dissolve as there were many white crystals still present. I then tried dissolving the granules in ~10 ml of 96% Isopropyl alcohol and then added 90 ml warm distilled water with the same results, many white crystals present.
The chemical is from Thomas Scientific and information is as follows:
CAS#:298-96-4
Form: solid
Formula: C19H15CIN4
Formula Weight: 334.80
Sensitivity 1: Hygroscopic
Sensitivity 2: Light sensitive

Any help would be appreciated.

Offline Hunter2

  • Sr. Member
  • *****
  • Posts: 2311
  • Mole Snacks: +190/-50
  • Gender: Male
  • Vena Lausa moris pax drux bis totis
Re: problems dissolving tetrazolium chloride granules
« Reply #1 on: November 06, 2024, 10:52:13 AM »
Do you have the right chemical. The solubility is about 50 g/l. Should be easy to dissolve 1 g in 100 ml

Sponsored Links