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Topic: Please help me!  (Read 6985 times)

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Offline kemi1

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Please help me!
« on: December 05, 2006, 05:03:45 AM »
Hi everybody :)
I am new here. I want to ask you. I hope you can help me.  Why do we use p-chloromercuribenzoate-agarose affinity column( if I want to purify an enzyme)?
Thank you!
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Offline Albert

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Re: Please help me!
« Reply #1 on: December 05, 2006, 06:56:16 AM »
What is unclear: afinity chromatography or p-chloromercuribenzoate? The latter easily bind to the matrix (yielding HCl) and the -COOH group on the other end of the molecule is a common one when it comes to immobilise a protein.

For the former, check this out: http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/A/AffinityChrom.html

Short but clear, I believe.
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Offline Borek

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Re: Please help me!
« Reply #2 on: December 05, 2006, 07:16:39 AM »
Quote from: kemi1 on December 05, 2006, 05:03:45 AM
I am new here.

Please read forum rules. Don't cross post.
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