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Topic: Gel electrophoretic separation of DNA  (Read 6269 times)

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Offline Snake

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Gel electrophoretic separation of DNA
« on: February 17, 2007, 05:23:12 AM »
hey guys, does anyone know what will happen if i prepared the agarose gel with water instead of using the buffer to make it?

the agarose gel is used to in electrophorsis to separate the DNA.

Offline yolop

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Re: Gel electrophoretic separation of DNA
« Reply #1 on: February 17, 2007, 08:43:38 AM »
Yeah, when you run the gel it will fal apart.

Offline Yggdrasil

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Re: Gel electrophoretic separation of DNA
« Reply #2 on: February 18, 2007, 05:46:32 PM »
The ions in the buffer allow the gel to conduct electricity.

Offline Bronwen Dekker

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Re: Gel electrophoretic separation of DNA
« Reply #3 on: February 20, 2007, 05:20:52 AM »
Is there a biological / scientific question that you would like to answer by not using the normal electrophoresis buffer? The reason I ask, is that it might be that you could get the question answered by using a different buffer / electrophoretic conditions / different separation method.
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Offline chiralic

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Re: Gel electrophoretic separation of DNA
« Reply #4 on: February 22, 2007, 06:17:42 PM »
Quote
what will happen if i prepared the agarose gel with water instead of using the buffer to make it?

Quote
Is there a biological / scientific question that you would like to answer by not using the normal electrophoresis buffer? The reason I ask, is that it might be that you could get the question answered by using a different buffer / electrophoretic conditions / different separation method.


Gels made in water will solidify clearer than gels made with buffer

Offline Bronwen Dekker

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Re: Gel electrophoretic separation of DNA
« Reply #5 on: February 24, 2007, 05:47:12 AM »
When I was talking about answering a scientific question, I meant a scientific question regarding the DNA rather than the gel.  :)

... and the specific question that came to mind related to the 'strandness' of the DNA, i.e. is it single or double-stranded...

See: http://www.natureprotocols.com/2007/01/25/twodimensional_strandnessdepen.php for an example.

I realise that it is probably a sidetrack from the original question, but thought there might have had a reason for wanting to try different separation conditions.
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