okay...so i have to determine the concentration of riboflavin from a fluorescent spectrophotometric methods....and so far i've got the Wavelength (544.29 nm), i've got the signal run values (too many numbers to plug in), and i've concentrations; and now i've got to make my calibration curve
now....when i make my calibration curve for fluorescent signal intensity vs. concentration, how do i figure out concentration???
most importantly, what does the slope of the calibration curve for this graph give me???