[augustfan]

I am trying some of my past exam questions, I have a lot of questions.

I don't know how to separate mRNAs from Total RNAs.

and there is one more question that I could not even understand its idea.


I will update this thread in fixed time interval.

Thanks all who can, and tried to asnwer and help me!

[augustfan]

Also, what is Amplification Refractory Mutation System? In its use in PCR system, how do it works?

[Equi]

I don't know how to separate mRNAs from Total RNAs.

Most mRNAs have a polyA tail and therefore bind quite happily to a oligo-dT column (affinity chromatography), while the rest of the RNAs can be found in the flow-through.

[augustfan]

In a question from my pastpapers, it is mentioned that there is a possibilities to detect, by PCR, different kinds of mutations, it stress the absence of need to do any post - PCR modification or analysis.

Is it really possible?

[Einherjar_PT]

I don't know many about PCR techniques and protocols (just the regular stuff), but I think there are some PCR techniques that detect point mutations like those (I mean yes, that is possible). Maybe implying fluorescent markers... . Can someone bring some light to the subject??