October 18, 2021, 01:06:04 PM
Forum Rules: Read This Before Posting


Topic: x ray development  (Read 2450 times)

0 Members and 1 Guest are viewing this topic.

Offline shininglight

  • New Member
  • **
  • Posts: 3
  • Mole Snacks: +0/-0
x ray development
« on: August 14, 2007, 07:06:59 AM »
hi, could someone explain how after a western blot is treated with substrate it is developed in dark room with x ray film and where the antibody attached to protein on the membrane a black spot appears , i know the procedure i just dont understand how it happens, thanks

Offline sdekivit

  • Chemist
  • Full Member
  • *
  • Posts: 403
  • Mole Snacks: +32/-3
  • Gender: Male
  • B.Sc Biomedical Sciences, Utrecht University
Re: x ray development
« Reply #1 on: August 14, 2007, 02:03:41 PM »
after transferring the proteins to the sheet antibodies (primary antibody) is incubated that specifically binds to the protein pof interest.

next a second antibody directed against the constant region of the primary antibody (say the first antibody is derived from a mouse, the second antibody derived from a goat is directed to mouse antibodies)

--> the second antibody is conjugated with a radioactive label that can be made visible on an autoradiogram (x-ray film)


Sponsored Links