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physical properties of THP for HPLC start up

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Offline Edward

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« on: August 24, 2007, 08:57:40 PM »
I urgently need to know the physical properties of THP including acidity, pKa, solubility, structure, polarity to start an HPLC exercise.  Is THP able to be extracted by Varian Tox A and Tox B LLE?  What is the rationale for this LLE with reference to its physico-chemical property?  Is a reversed phase C18 sufficient? I read from paper that a gradient elution generated by proportional mixing of solvent A:50mL/L acetonitrile, and solvent B 500mL/L, both in 50mL/L phosphate buffer, pH 3.0, containing 3mL/L triethylamine and 375mg/L SOS, was maintained at 12% for 3min, and returned to 12% in 3min, the waiting time between analytical runs was 7 min.  The flow rate was 1.0mL/min.  
I am new to HPLC and don't know how the author derived at this.  Could anyone pls advise how optimization can be done?
For UV detection, is it beneficial to run a UV spectrum just by scanning a diluted commercial THP?
Thank you very much
« Last Edit: August 24, 2007, 09:29:55 PM by Edward »

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