Who is “Lineburke
”?, - never heard of him/it, and it seems nobody (Google, Yahoo!, MSN, Alta Vista, etc.) else too!
Abs/min indicates a “Single Wavelength measurement
) for a Reaction, what seems to be not very wisely!But let’s do the little “brain acrobatics” anyway:
” or more correct ΔAbs/Δt
, like dAbs/dt
is a “Reaction Rate
”, and if you use the “Special Beer-Lambert Law
” (for only a SWM
it’s enough) you have to write:
ΔAbs/Δt ==> ΔAλ/Δt = Δ(ελ * c * d)/Δt
On the other side and from the definition of ελ
If you set for your case for Aλ
= 2500 AU into the “Special Beer-Lambert Law
”: Aλ = ελ * c * d
you will result in:
2500 AU = 2500 L mole-1 cm-1 * c * dOr with simple words: ελ
” the theoretical Absorbance
(value) for a Solution with Concentration = 1.00 Mole/L measured in a 1.00cm Cuvette with the given ελ
. And the final calculation:
2500 AU/min = 1.00 mole/min ===> 0.429AU/min = mole/min.
(I’m still searching for its real sense.)
Could this be of help to you?
What should in this case “Vmax” and “Km” tell us?