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Topic: calculating pka from absorbance  (Read 7788 times)

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  • Guest
calculating pka from absorbance
« on: February 01, 2005, 07:40:23 PM »
im trying to calculate the pka of  based on experimental data in the form of absorbance at 450nm. the absorbance naturally changes with pH.

I have been trying to manipulate Beer's Law and the H-H buffer equation to awnser this question, but to no avail.


  • Guest
Re:calculating pka from absorbance
« Reply #1 on: February 01, 2005, 11:29:06 PM »
Beer's law only hold true between specific concentrations (ie absorbances). Maybe you're outside the range?

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