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calculating pka from absorbance
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billitar:
im trying to calculate the pka of based on experimental data in the form of absorbance at 450nm. the absorbance naturally changes with pH.
I have been trying to manipulate Beer's Law and the H-H buffer equation to awnser this question, but to no avail.
Tetrahedrite:
Beer's law only hold true between specific concentrations (ie absorbances). Maybe you're outside the range?
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